This Trophy requires the player to rescue the Little Sister without destroying any of the Turrets in the stage: make extensive use of the Target DummyPlasmid to draw all of the Turret fire during your first journey through the Firing Range.
This Trophy requires the player only to find the four Roses hidden throughout the stage. Their locations are:
On the desk in the Reception.
Hanging from the ceiling in the Machine Room; once one has Winter Blast 3 lure the Bouncer close to one of the elevated areas, freeze him, and jump on top of him. From here, the player should be able to reach the Rose if they jump.
Inside the central RPG Turret; once the Bouncer guarding the Little Sister is dead, search his corpse for a Grenade Launcher and ten Frag Grenades. Destroy the middle RPG Turret and knock it down to the ground, where the player can search it for the third Rose.
Behind a secret door in the Machine Room; it opens when the Bouncer in the Pressure Plate Room stands on the pressure plate. Lure him onto the plate using Target Dummy and run towards the Machine Room as quickly as possible. Grab the Rose before the Bouncer can move off the plate.
Like all of the Challenge Rooms, the Little Sister the player must save appears to have already been "rescued", as she bears none of the signs of being implanted with a Sea Slug.
When collecting the Rose from the RPG Turret in the PlayStation 3 version of the content, an image icon of a Rose will appear in the Turret's inventory. However, in the Xbox 360 version, the image icon will be an Apple, as seen from the EVE Upgrade slot.